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anti-islet-1 homeobox  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank anti-islet-1 homeobox
    Anti Islet 1 Homeobox, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+islet+1+antibodies/anti-Islet-1+homeobox/custom%4040%2E2d6%4042269606
    Average 95 stars, based on 162 article reviews
    anti-islet-1 homeobox - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Transmembrane voltage potential controls embryonic eye patterning in Xenopus laevis
    Article Snippet: The XAP2 and anti-Islet-1 antibodies developed by D. S. Sakaguchi and W. A. Harris and by T. M. Jessell and S. Brenner-Morton, respectively, were obtained from the Developmental Studies Hybridoma Bank (DSHB) developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biology, Iowa City, IA 52242, USA.

    Incubation:

    Article Title: Tissues exhibiting inhibitory [correction of inhibiory] and repulsive activities during the initial stages of neurite outgrowth from the dorsal root ganglion in the chick embryo.
    Article Snippet: .. The cultures were incubated overnight with the polyclonal anti-SC2/axonin-1 antibody which has been shown to stain DRG axons (Tanaka and Obata, 1984; Sakurai et al., 1994), then with a biotinylated secondary anti-rabbit IgG antibody (Vector) for 4–5 h, followed by the peroxidase-conjugated avidin– biotin complex (Vector) for 2–3 h. In some cases, anti-b-tubulin (Yaginuma et al., 1990) and anti-Islet-1 antibodies (Developmental Studies Hybridoma Bank, 40.2D6; Ericson et al., 1992) were used as the primary antibodies to label axons and cells in the dorsal spinal cord, respectively. ..

    Staining:

    Article Title: Tissues exhibiting inhibitory [correction of inhibiory] and repulsive activities during the initial stages of neurite outgrowth from the dorsal root ganglion in the chick embryo.
    Article Snippet: .. The cultures were incubated overnight with the polyclonal anti-SC2/axonin-1 antibody which has been shown to stain DRG axons (Tanaka and Obata, 1984; Sakurai et al., 1994), then with a biotinylated secondary anti-rabbit IgG antibody (Vector) for 4–5 h, followed by the peroxidase-conjugated avidin– biotin complex (Vector) for 2–3 h. In some cases, anti-b-tubulin (Yaginuma et al., 1990) and anti-Islet-1 antibodies (Developmental Studies Hybridoma Bank, 40.2D6; Ericson et al., 1992) were used as the primary antibodies to label axons and cells in the dorsal spinal cord, respectively. ..

    Avidin-Biotin Assay:

    Article Title: Tissues exhibiting inhibitory [correction of inhibiory] and repulsive activities during the initial stages of neurite outgrowth from the dorsal root ganglion in the chick embryo.
    Article Snippet: .. The cultures were incubated overnight with the polyclonal anti-SC2/axonin-1 antibody which has been shown to stain DRG axons (Tanaka and Obata, 1984; Sakurai et al., 1994), then with a biotinylated secondary anti-rabbit IgG antibody (Vector) for 4–5 h, followed by the peroxidase-conjugated avidin– biotin complex (Vector) for 2–3 h. In some cases, anti-b-tubulin (Yaginuma et al., 1990) and anti-Islet-1 antibodies (Developmental Studies Hybridoma Bank, 40.2D6; Ericson et al., 1992) were used as the primary antibodies to label axons and cells in the dorsal spinal cord, respectively. ..

    Immunohistochemistry:

    Article Title: Mice deficient in the chemokine receptor CXCR4 exhibit impaired limb innervation and myogenesis.
    Article Snippet: Institute of Anatomy, University of Leipzig, Medical Faculty, Liebigstr.. 13, 04103 Leipzig, Germany Institute of Anatomy, Anatomy & Cell Biology, University of Bonn, 53115 Bonn, Germany Department of Anatomy and Cell Biology, University of Ulm, 89069 Ulm, Germany Department of Pharmacology and Toxicology, University of Ulm, 89069 Ulm, Germany Howard Hughes Medical Institute, Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, NY 10016, USA

    Activity Assay:

    Article Title: Mice deficient in the chemokine receptor CXCR4 exhibit impaired limb innervation and myogenesis.
    Article Snippet: Institute of Anatomy, University of Leipzig, Medical Faculty, Liebigstr.. 13, 04103 Leipzig, Germany Institute of Anatomy, Anatomy & Cell Biology, University of Bonn, 53115 Bonn, Germany Department of Anatomy and Cell Biology, University of Ulm, 89069 Ulm, Germany Department of Pharmacology and Toxicology, University of Ulm, 89069 Ulm, Germany Howard Hughes Medical Institute, Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, NY 10016, USA

    Binding Assay:

    Article Title: Mice deficient in the chemokine receptor CXCR4 exhibit impaired limb innervation and myogenesis.
    Article Snippet: Institute of Anatomy, University of Leipzig, Medical Faculty, Liebigstr.. 13, 04103 Leipzig, Germany Institute of Anatomy, Anatomy & Cell Biology, University of Bonn, 53115 Bonn, Germany Department of Anatomy and Cell Biology, University of Ulm, 89069 Ulm, Germany Department of Pharmacology and Toxicology, University of Ulm, 89069 Ulm, Germany Howard Hughes Medical Institute, Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, NY 10016, USA



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    Image Search Results


    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Journal: bioRxiv

    Article Title: Engineering a pacemaker-driven human mini-heart guided by spatial and single cell multi-omics of sinoatrial node development

    doi: 10.64898/2026.05.07.723626

    Figure Lengend Snippet: a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Article Snippet: At 54 hpf larvae were fixed in 2% PFA for 2 hours at room temperature, washed extensively in PBS with 0.3% TritonX-100 (PBX), incubated in blocking buffer (BB: PBX, 0.5% BSA, 10% goat serum) for 2 hours at room temperature, incubated overnight at 4°C in BB containing 1/50 dilution of mouse anti-ISL1/2 antibody (Iowa Developmental Studies Hybridoma Bank #39.4D5), washed extensively with PBX, incubated at room temperature for 2 hours in BB with a 1/200 dilution of goat anti-mouse IgG2b Alexa Fluor568 (Invitrogen #A-21144), washed extensively with PBX, and mounted in 1:1 PBS:glycerol for imaging on a Zeiss LSM800 confocal microscope.

    Techniques: Fluorescence, Control, Staining

    (A) Schematics showing the location and signalling requirements of NMP and pLPM progenitors in the mouse E8.5 embryo (left) and the treatment conditions used to induce NMPs and pLPM cells from hPSCs (right). (B) RT-qPCR-based expression analysis of key NMP and pLPM markers after 3 days of iPSC differentiation toward NMPs in the presence and absence of BMP4 ( n = 3 independent experiments). Relative expression levels to iPSCs were normalised to GAPDH gene expression (* p<0.05, ** p<0.01, *** p<0.001, mean ±SD) (paired, two-tailed t -test). (C) Immunofluorescence analysis of the expression of TBXT and ISL1 on day 3 of differentiation. Scale bars represent 100 µm. Image analysis of the percentage of nuclei positive for TBXT and ISL1 protein expression is also shown. Graph shows mean values ( n = 3 independent experiments) (* p<0.05, ** p<0.01, *** p<0.001, mean ±SD) (unpaired t -test with Welch’s correction). (D) Heatmap of differentially expressed genes showing key NMP and pLPM/HGTp markers from bulk RNA-seq analysis on day 3. Expression values are shown as Z-scores across samples. (E) Heatmap of differentially expressed genes showing KEGG signalling components on day 3. Expression values are shown as Z-scores across samples.

    Journal: bioRxiv

    Article Title: Generation of human hindlimb/genital tubercle progenitors from pluripotent stem cells

    doi: 10.64898/2026.04.14.718471

    Figure Lengend Snippet: (A) Schematics showing the location and signalling requirements of NMP and pLPM progenitors in the mouse E8.5 embryo (left) and the treatment conditions used to induce NMPs and pLPM cells from hPSCs (right). (B) RT-qPCR-based expression analysis of key NMP and pLPM markers after 3 days of iPSC differentiation toward NMPs in the presence and absence of BMP4 ( n = 3 independent experiments). Relative expression levels to iPSCs were normalised to GAPDH gene expression (* p<0.05, ** p<0.01, *** p<0.001, mean ±SD) (paired, two-tailed t -test). (C) Immunofluorescence analysis of the expression of TBXT and ISL1 on day 3 of differentiation. Scale bars represent 100 µm. Image analysis of the percentage of nuclei positive for TBXT and ISL1 protein expression is also shown. Graph shows mean values ( n = 3 independent experiments) (* p<0.05, ** p<0.01, *** p<0.001, mean ±SD) (unpaired t -test with Welch’s correction). (D) Heatmap of differentially expressed genes showing key NMP and pLPM/HGTp markers from bulk RNA-seq analysis on day 3. Expression values are shown as Z-scores across samples. (E) Heatmap of differentially expressed genes showing KEGG signalling components on day 3. Expression values are shown as Z-scores across samples.

    Article Snippet: Following primary antibodies were used; TBXT (Abcam, ab209665, 1:1000), ISL1 (DSHB, 39.4D5, 1:200), HAND1 (R&D Systems, AF3168, 1:100), PITX1 (Novus Bio, NBP1-88644, 1:500), HOXC9 (Abcam, ab50839, 1:200), KDR (R&D Systems, AF357, 1:1000), TP63 (Abcam, ab124762, 1:200).

    Techniques: Quantitative RT-PCR, Expressing, Gene Expression, Two Tailed Test, Immunofluorescence, RNA Sequencing

    (A) UMAP visualisation of sc-RNA seq data from late RA treated cells collected on day 10 of differentiation. Cells were divided into 3 clusters indicated by different colours. Below: Bar graph showing the percentage of total cells per cluster. (B) Stacked violin plots showing the expression (log-normalized) of key lineage markers enriched across identified cell clusters. (C) Dot plot showing the proportion of cells per cluster expressing the indicated marker genes. The colour bar indicates the average log-normalized expression values. (D, E) Feature UMAPs showing the expression of representative marker genes (log-normalized). (F) UMAP showing the proportions of ISL+/TBX5+/TBX4+ cells across the identified cell clusters. Below: Bar graph showing the percentage of cells per cluster calculated for each cluster. (G) Dot plot showing the percentage expression of key surface ectoderm markers for each cell cluster. The colour bar indicates the average log-normalized expression values. (H) Immunofluorescence analysis of the expression of TP63 and ISL1 on day 10 HGTps. Sequential z-stacks are shown (z=1 bottom, z=20 top). Scale bars represent 100 µm.

    Journal: bioRxiv

    Article Title: Generation of human hindlimb/genital tubercle progenitors from pluripotent stem cells

    doi: 10.64898/2026.04.14.718471

    Figure Lengend Snippet: (A) UMAP visualisation of sc-RNA seq data from late RA treated cells collected on day 10 of differentiation. Cells were divided into 3 clusters indicated by different colours. Below: Bar graph showing the percentage of total cells per cluster. (B) Stacked violin plots showing the expression (log-normalized) of key lineage markers enriched across identified cell clusters. (C) Dot plot showing the proportion of cells per cluster expressing the indicated marker genes. The colour bar indicates the average log-normalized expression values. (D, E) Feature UMAPs showing the expression of representative marker genes (log-normalized). (F) UMAP showing the proportions of ISL+/TBX5+/TBX4+ cells across the identified cell clusters. Below: Bar graph showing the percentage of cells per cluster calculated for each cluster. (G) Dot plot showing the percentage expression of key surface ectoderm markers for each cell cluster. The colour bar indicates the average log-normalized expression values. (H) Immunofluorescence analysis of the expression of TP63 and ISL1 on day 10 HGTps. Sequential z-stacks are shown (z=1 bottom, z=20 top). Scale bars represent 100 µm.

    Article Snippet: Following primary antibodies were used; TBXT (Abcam, ab209665, 1:1000), ISL1 (DSHB, 39.4D5, 1:200), HAND1 (R&D Systems, AF3168, 1:100), PITX1 (Novus Bio, NBP1-88644, 1:500), HOXC9 (Abcam, ab50839, 1:200), KDR (R&D Systems, AF357, 1:1000), TP63 (Abcam, ab124762, 1:200).

    Techniques: RNA Sequencing, Expressing, Marker, Immunofluorescence